DNA molecules between telepathy

The latest study, DNA molecules appear to have telepathy. Scientists have discovered the double helix structure of DNA molecules to identify themselves with the "matching" elements, even some distance away, on the surface and no other outside help, the match of the two elements together to the end. In accordance with the previous understanding of DNA, scientists study the double helix structure of DNA molecules are arranged in accordance with the laws of their own. Helix structure of DNA molecule is composed of many ODN from the polymerization of long-chain, as the composition of the DNA base pairs of only four: adenine (A), guanine (G), cytosine (C) And thymine (T), therefore, there are four types of DNA, we usually A, T, C, G Four alphabet tag them, and use of chemical methods to match their children - A equipped with T, C equipped with G . In fact, the well-known "to exchange the role of the base together" is not the DNA double helix molecule in close connection with the bodies of the root causes. The DNA double helix structure was so stable, because the outside of deoxyribose phosphate and arranged in alternating the basic framework, the inside of the base to form hydrogen bonds through the base right. Scientists study the mixture through the fluorescence of this double-stranded DNA molecule structure. These DNA molecules were placed on some of the salt water, salt water test does not contain any proteins, DNA molecules will enable non-binding, as well as any material that may affect the trial. Strangely enough, together with the same number of base pairs of DNA molecules is the other remaining twice the number of DNA molecules. Even though they look like a very strange, like a psychological sense, but in fact only a DNA molecule in the exercise under the laws of physics, not a supernatural phenomenon. Take charge of deoxyribose and phosphate arranged in turn composed of DNA molecules will be mutually exclusive, however, because of the DNA double helix structure of the special, making the repulsive force between them to reach the minimum. In order to understand more vividly the researchers said, let us try the double helix structure of DNA molecules into a corkscrew imagination to form a long chain of DNA molecules in the base of support outside the framework and the role of hydrogen bonds in the middle of, So that the screw cone to the direction of a distorted, twisted into a spiral, then the process will be part of the same degree of bending and other elements of the sunken part of the coordinated combination. Scientists point out that this "psychological sense" would help the DNA molecules in the chaos of their pre-arranged neatly, which can effectively avoid errors occur when the combination of DNA, it effectively avoiding cancer, aging and other diseases. However, due to the same DNA sequence in fact disrupt the combination of sexual reproduction is a meaningful, because of the need to ensure that future generations have the genetic diversity.

RNA extraction from tissues

Procedures for RNA extraction from tissues :

1. pre-weigh an empty cryovial
2. take the tissue specimen out from the cryovial and place it on a 100mm petri dish
3. use a scapel and sterican (like fork and knife) to cut the tissue into desired size
4. transfer the tissue into the pre-weigh cryovial and weigh again (this will give the exact weigh of the tissue being process)
5. transfer the surplus tissue into the original cryovial and keep in the nitrogen tank
6. add 650µl of RLT buffer into the cryovial
7. pound the tissue using a pipette tip until the solution turn orange in colour.
8. transfer the solution into QIA shredder column and centrifuge at 14 000rpm for 4minutes
9. transfer the flow through into a new eppendorf tube and add in 550µl of 70% ethanol
10. centrifuge the eppendorf tube at 14 000rpm for 3 minutes
11. transfer the solution into Rneasy column and centrifuge at 14 000rpm for 15 seconds
12. discard the flow through
13. add 700µl RW1 buffer into the Rneasy column and centrifuge at 14 000rpm for 15 seconds
14. discard the flow through
15. add 500µl RPE buffer into the Rneasy column and centrifuge at 14 000rpm for 15 seconds
16. discard the flow through
17. centrifuge the Rneasy column at 14 000rpm for 1 minute
18. transfer the Rneasy column into a new sterilised round bottom tube
19. add 30µl Rnase-free water and centrifuge at 14 000rpm for 1 minute
20. transfer the flow into the column and centrifuge at 14 000rpm for 1 minute
21. the flow through is the RNA extracted

Thing to note
- after taking the cryovial of tissue out from the nitrogen tank, keep it in liquid nitrogen.